Mapping the conformational energy landscape of Abl kinase using ClyA nanopore tweezers
نویسندگان
چکیده
Protein kinases play central role in cellular regulation by catalyzing the phosphorylation of target proteins involved complex physiological processes, achieved their structural flexibility switching between active state and inactive states. Quantitative characterization kinase conformational dynamics is important yet challenging. Nanopore analysis emerging as an attractive single-molecule tool for probing protein due to several advantages, such label-free, being able detect a wide range timescales (μs∼mins).
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2022
ISSN: ['0006-3495', '1542-0086']
DOI: https://doi.org/10.1016/j.bpj.2021.11.1084